Service Description:
Over the past 10 years, 16S/18S/ITS rRNA gene sequencing is the best tool to study bacterial and fungal taxonomy and molecular phylogeny.By taking advantage of PacBio SMRT long reads sequencing technology, Omics Drive can provide full-length 16S/18S/ITS rRNA sequencing service to help your research.
Take 16S rRNA as an example, the average read lengthof the PacBio SMRT sequences is 10~18 K, which can cover all variable areas of16S rRNA (the total length is around 1,500 bp). Meanwhile, circular consensus sequencing (CCS) produces high intra-molecular consensus accuracy for full-length 16S rDNA to achieve accurate to 搒pecies� classification and identification.
18S rRNA (total length 1,500-2,000 bp) and ITS (about400-900 bp) are also able to be performed by full-length sequencing for microbiome analysis.
Advantages:
Strain-level identification by the full length of amplicons
Bioinformatics Analysis:
OTUs clustering and filtering
OTUs analysis and species annotation
PCA,Venn diagram. A rank curve will be generated based on OTU abundance
Alpha diversity, Beta diversity, Meta-analysis
Multi-variate statistical analysis
Analysis Flow:
