Service Description:
Omics Drive provides PacBio Single Molecular Real-Time (SMRT) sequencing for microbial whole genome sequencing to get a comprehensive view of the microbial genome.
SMRT Sequencing has long reads (average >15,000 bp, some reads >100,000 bp) and the highest consensus accuracy. It is especially helpful for genome de novo assembly. As we know, repetitive stretches of DNA are abundant and are one of the main technical challenges that hinder accurate sequencing and genome assembly efforts. In the case of bacteria, the rRNA gene operon is often the largest region of repetitive sequence and range in size between 5 and 7 kb. Microbial whole genome sequencing by illumina HiSeq platforms utilizes sequencing by synthesis technology which is limited by its read length (50 to 300 bp), and as it requires PCR amplification of multiple DNA templates before sequencing which potentially increase base-composition bias.
With SMRT Sequencing on the Sequel System, there is no need sacrifice quality for afford ability when assembling bacterial genomes. Go well beyond draft-quality to achieve reference-quality assemblies,closing even the most repeat-dense and GC-rich genomes and resolving plasmids.
The advantage of SMRT long reads, which can overcome the problem of abnormal GC and high duplication of micorbial genomes, often assembled into a single contig. Among all assemblies, the PacBio assembly recovered the highest number of core and virulence proteins, and housekeeping genes based on whole-genome multilocus sequence typing.
Key Features and Advantages:
Generate platinum-standard, closed reference genomes
Affordably assemble gold-standardgenomes
Clarify the role of transposons,phage insertions, and other structural variants in the evolution of virulence
Recover plasmids to track drugresistance and transmission paths
Bioinformatics Analysis:
Data quality control
Assembly
Genome Annotation
Functional Annotation
Comparative Genomics
Evolutionary Analysis
...
(More analysis upon request)
Analysis Flow:
