Service Description:
Changes inisoform variants and relative abundancies are an important clue in deciphering how genomic variants drive the phenotypic differences between health and disease. Only long-read sequencing can provide unbiased, direct detection ofall the isoforms present in the samples. Iso-Seq analysis allowus to generate full-length cDNA sequences without assembly. It characterize transcript isoforms within targeted genes or across an entire transcriptome.
Key Features and Advantages:
Discover new genes, transcripts and alternative splicing events
Improve genome annotation to identify gene structure, regulatory elements, and coding regions
Increase the accuracy of RNA-seq quantification with isoform-level resolution
Sequencing Platforms:
PacBio Sequel
Bioinformatics Analysis:
Data Quality Control
Annotation of Gene Function, CDS Prediction and SSR Detection
Expression and Differential Expression
GO and KEGG
...
(More upon request)
Analysis Flow:
