Service Description:
Changes in isoform variants and relative abundancies are an important clue in deciphering how genomic variants drive the phenotypic differences between health anddisease. Only long-read sequencing can provide unbiased, direct detection of all the isoforms present in the samples.
Iso-Seq analysis allowus to generate full length cDNA sequences without assembly. It characterize transcript isoforms within targeted genes or across an entire transcriptome
Advantages of Iso-seq:
Discover new genes, transcripts and alternative splicing events
Improve genome annotation to identify gene structure, regulatory elements, and coding regions
Increase the accuracy of RNA-seq quantification with isoform-level resolution
Sequencing Platforms:
PacBio Sequel
Bioinformatics Analysis:
Data quality control
Annotation of gene Function, CDS prediction and SSR detection
Expression and differential expression
GO and KEGG
Analysis Flow:
