Strand-Specific RNA Sequencing
Service Description:
Strand specific libraries allow us to identify the coding DNA strand for the transcript. It enables to get precise measurement of mRNA strand orientation to enhance transcript annotation, and increase alignment efficiency. It also enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Sequencing Platforms:
HiSeq/Novaseq
Bioinformatics Analysis:
Data quality control
Mapping reads to reference genome
Gene expression quantification
Correlation analysis and differential expression analysis
GO enrichment analysis of differentially expressed genes
KEGG pathway enrichment analysis
...
(More analysis upon request)
Analysis Flow:
