Service Description:
CircRNA are characterized by covalently closed loop feature thus lacking 5� end caps and 3� poly-A tails. It is a novel member of the non-coding RNAs (ncRNAs), generated by non-sequential back splicing of exons, introns or both.
When first identified in the 1970s, circRNAs were regarded as viral genomes or by products of mis-splicing events. But recent researches have revealed that circRNAs are evolutionarily conserved across plants, animals, and human beings, with important biological functions.
CircRNAs are highly stable forms of ncRNAs dueto their nuclease resistance properties.
Advantagesof CircRNA Sequencing:
Identifies known and novel circRNAs
Allows profiling of circRNAs across a wide dynamic ranges
Discover novel biomarkers and circRNAs regulatory networks
Sequencing Platforms:
HiSeq/Novaseq
Bioinformatics Analysis:
Data quality control
CircRNAs identification
Expression and differential expression of CircRNAs
Enrichment analysis of the source genes of differential CircRNAs
Discover miRNA trargets from CircRNAs
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(More analysis upon request)
Analysis Flow:
