Service Description:
Microbial whole genome sequencing is important for precisemicrobial identification, the generation of complete reference genomes (denovo sequencing), comparative genomic studies (re-sequencing), and genomic exploitation. Comparative genomic studies can identify individual genetic variations and large-scale structural variations within a populationfor which a reference genome is available. Evolutionary characteristics andphylogenetic relationships can be hence inferred. Microbial whole genomesequencing provides the possibility of gene finding and annotation. After multiple genes are explained, novel biochemical pathways that may be beneficial for medicine and biotechnology will likely be identified.
Advantages:
A time-effective and cost-efficient approach;
Broad applications: de novo sequencing, gene annotations,comparative genomic studies, evolutionary studies of micro-organisms;
Drug discovery and development: assess the contribution of DNA on pathogenesis, and understand the role of mobile elements in drug resistance and transmission;
Bioinformatics Analysis:
For de novo
Data quality control
Genome advanced assemble
Genome survey: genome size, GC distribution, repeat rate
Genome component analysis: gene structure prediction, repeat sequences, non-codingRNAs
Gene function annotation
For requencing
Data quality control
Alignment with reference
SNP/InDel calling, annotation and statistics
SVcalling, annotation and statistics
Analysis Flow:
