Service Description:
Metatranscriptomics is the culture-independent profiling (including protein-coding and non-coding DNA) of microbial community-wide gene expression, which is capable of monitoring RNA-based regulation and expressed biological signatures of complex bacterial communities in a given sample at a given moment and under specific conditions. It elucidates three aspects of a microbial community, including gene activity diversity, gene expression abundance, and differential gene expression analysis. The gene expression analysis can tell which genes exhibit the highest change in expression levels in different conditions potentially to identify biomarkers and expression signatures.
Advantages:
Aculture-free method to reveal the true extent of microbial diversity
Permitting function-based activity screens
More targeted than shotgun random sequencing
Cost-efficient and time-effective
Bioinformatics Analysis:
Data quality control
Statistics analysis of data production and quality
De novo assembly
Gene functional annotation
rRNA& mRNA taxonomic analysis
Gene expression analysis
Differential expression analysis
GO enrichment analysis of differentially expressed genes (DEGs)
KEGG pathway enrichment analysis of differentially expressed genes(DEGs)
Comparative analysis between various samples
...
(More analysis upon request)
Analysis Flow
