Service Description:
ChIP-Seq (chromatin immunoprecipitation sequencing) is a technique which combines chromatin immunoprecipitation (ChIP) with NGS for identifying genome-wide DNA binding sites for transcription factors and other proteins. It could be used to determine how transcription factors and other chromatin-related proteins affect phenotypic mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential to investigate biological processes and disease states. ChIP-Seq has played important roles in applications include studies on gene regulation, transcription complex assembly, DNA repair, histone modification, developmental mechanisms, disease progression and modifications.
Advantages of ChIP-Seq:
Captures DNA targets for transcription factors or histone modifications across the entire genome of any organism
Defines transcription factor binding sites
Reveals gene regulatory networks in combination with RNA sequencing and methylation analysis
Utilizing widely accepted software and latest programs for motif prediction, peak annotation, functional analysis and data visualization of ChIP-Seq
Bioinformatics Analysis:
Data quality control
Mapping onto reference genome
Peak calling
Motif prediction
Peak annotation (downstream or overlapping gene, TSS)
Functional analysis of peak-associated genes (Gene Ontology, pathway)
Visualization of ChIP-seq data
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(More analysis upon request)
Analysis Flow:
